Format

Send to

Choose Destination
Elife. 2015 May 15;4. pii: e06516. doi: 10.7554/eLife.06516.

Catastrophic chromosomal restructuring during genome elimination in plants.

Author information

1
Department of Plant Biology, University of California, Davis, Davis, United States.
2
Genome Center, University of California, Davis, Davis, United States.
3
School of Biology, Indian Institute of Science Education and Research, Thiruvananthapuram, India.
4
Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
5
Department of Molecular and Cellular Biology, University of California, Davis, Davis, United States.
6
Gordon and Betty Moore Foundation, Howard Hughes Medical Institute, University of California, Davis, Davis, United States.

Abstract

Genome instability is associated with mitotic errors and cancer. This phenomenon can lead to deleterious rearrangements, but also genetic novelty, and many questions regarding its genesis, fate and evolutionary role remain unanswered. Here, we describe extreme chromosomal restructuring during genome elimination, a process resulting from hybridization of Arabidopsis plants expressing different centromere histones H3. Shattered chromosomes are formed from the genome of the haploid inducer, consistent with genomic catastrophes affecting a single, laggard chromosome compartmentalized within a micronucleus. Analysis of breakpoint junctions implicates breaks followed by repair through non-homologous end joining (NHEJ) or stalled fork repair. Furthermore, mutation of required NHEJ factor DNA Ligase 4 results in enhanced haploid recovery. Lastly, heritability and stability of a rearranged chromosome suggest a potential for enduring genomic novelty. These findings provide a tractable, natural system towards investigating the causes and mechanisms of complex genomic rearrangements similar to those associated with several human disorders.

KEYWORDS:

arabidopsis; chromosome segregation; chromosomes; genes; genome instability; mitosis

PMID:
25977984
PMCID:
PMC4461816
DOI:
10.7554/eLife.06516
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for eLife Sciences Publications, Ltd Icon for PubMed Central
Loading ...
Support Center