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Food Chem. 2015 Nov 15;187:437-43. doi: 10.1016/j.foodchem.2015.04.111. Epub 2015 Apr 24.

Modifying glycoalkaloid content in transgenic potato--Metabolome impacts.

Author information

1
Environmental and Biochemical Sciences Group, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom. Electronic address: louise.shepherd@hutton.ac.uk.
2
Biomathematics and Statistics Scotland, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom.
3
Environmental and Biochemical Sciences Group, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom.
4
Environmental and Biochemical Sciences Group, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom; School of Life Sciences, Heriot-Watt University, Edinburgh EH14 4AS, Scotland, United Kingdom.
5
USDA, Agricultural Research Service, Crop Improvement and Genetics, Western Regional Research Centre, 800 Buchanan St, Albany, CA 94710-1105, USA.

Abstract

Metabolite profiling has been used to assess the potential for unintended composition changes in potato (Solanum tuberosum L. cv. Desirée) tubers, which have been genetically modified (GM) to reduce glycoalkaloid content, via the independent down-regulation of three genes SGT1, SGT2 and SGT3 known to be involved in glycoalkaloid biosynthesis. Differences between the three groups of antisense lines and control lines were assessed using liquid chromatography-mass spectrometry (LC-MS) and gas chromatography (GC)-MS, and data analysed using principal component analysis and analysis of variance. Compared with the wild-type (WT) control, LC-MS revealed not only the expected changes in specific glycoalkaloid levels in the GM lines, but also significant changes in several other metabolites, some of which were explicable in terms of known pathways. Analysis of polar and non-polar metabolites by GC-MS revealed other significant (unintended) differences between SGT lines and the WT, but also between the WT control and other control lines used.

KEYWORDS:

Genetic modification; Glycoalkaloids; Metabolomics; Potato (Solanum tuberosum L.); SGT; Transgenic

PMID:
25977048
DOI:
10.1016/j.foodchem.2015.04.111
[Indexed for MEDLINE]

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