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J Fish Biol. 2015 Jul;87(1):115-22. doi: 10.1111/jfb.12700. Epub 2015 May 14.

Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus.

Author information

1
Key Laboratory of Experimental Marine Biology, National & Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, Shandong, People's Republic of China.
2
University of Chinese Academy of Sciences, Beijing 10049, People's Republic of China.
3
Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shandong, People's Republic of China.

Abstract

A new brain-cell line derived from Japanese flounder Paralichthys olivaceus (POBC) was established. POBC was subcultured for 67 passages over the course of 420 days. The cultured cells were primarily epithelioid-like. Chromosome analysis revealed the cell line to possess the normal P. olivaceus diploid karyotype of 2n = 48t (telocentric chromosomes). The cells exhibited the astrocyte marker glial fibrillary acidic protein by immunocytochemistry, and significant fluorescent signals were observed when the cells were transfected with green fluorescent protein reporter plasmid. The established POBC would be ideal material for the study of function of fish ependyma, the central neuroendocrine system and endocrine disruptors in the marine environment.

KEYWORDS:

astrocyte; brain-cell line; epithelioid-like; karyotype; transfection

PMID:
25974130
DOI:
10.1111/jfb.12700
[Indexed for MEDLINE]

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