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J Appl Microbiol. 2015 Aug;119(2):400-10. doi: 10.1111/jam.12842. Epub 2015 Jun 24.

Pseudomonas moraviensis subsp. stanleyae, a bacterial endophyte of hyperaccumulator Stanleya pinnata, is capable of efficient selenite reduction to elemental selenium under aerobic conditions.

Author information

1
Biology Department, Colorado State University, Fort Collins, CO, USA.
2
UNESCO-IHE Institute for Water Education, Delft, The Netherlands.
3
Université Paris-Est, Laboratoire Géomatériaux et Environnement, UPEM, Marne-la-Vallée, Cedex 2, France.
4
Chemistry Department, Colorado State University, Fort Collins, CO, USA.
5
VIB Department of Structural Biology, Department of Bioengineering Sciences, Research Group Microbiology, Vrije Universiteit, Brussels, Belgium.
6
Plant-Microbe Interactions, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.
7
USDA-ARS, Fort Collins, CO, USA.
8
Biochemistry and Microbiology Department, Institute of Chemical Technology in Prague, Prague, Czech Republic.

Abstract

AIMS:

To identify bacteria with high selenium tolerance and reduction capacity for bioremediation of wastewater and nanoselenium particle production.

METHODS AND RESULTS:

A bacterial endophyte was isolated from the selenium hyperaccumulator Stanleya pinnata (Brassicaceae) growing on seleniferous soils in Colorado, USA. Based on fatty acid methyl ester analysis and multi-locus sequence analysis (MLSA) using 16S rRNA, gyrB, rpoB and rpoD genes, the isolate was identified as a subspecies of Pseudomonas moraviensis (97.3% nucleotide identity) and named P. moraviensis stanleyae. The isolate exhibited extreme tolerance to SeO3(2-) (up to 120 mmol l(-1)) and SeO4(2-) (>150 mmol l(-1)). Selenium oxyanion removal from growth medium was measured by microchip capillary electrophoresis (detection limit 95 nmol l(-1) for SeO3(2-) and 13 nmol l(-1) for SeO4(2-)). Within 48 h, P. moraviensis stanleyae aerobically reduced SeO3(2-) to red Se(0) from 10 mmol l(-1) to below the detection limit (removal rate 0.27 mmol h(-1) at 30 °C); anaerobic SeO3(2-) removal was slower. No SeO4(2-) removal was observed. Pseudomonas moraviensis stanleyae stimulated the growth of crop species Brassica juncea by 70% with no significant effect on Se accumulation.

CONCLUSIONS:

Pseudomonas moraviensis stanleyae can tolerate extreme levels of selenate and selenite and can deplete high levels of selenite under aerobic and anaerobic conditions.

SIGNIFICANCE AND IMPACT OF THE STUDY:

Pseudomonas moraviensis subsp. stanleyae may be useful for stimulating plant growth and for the treatment of Se-laden wastewater.

KEYWORDS:

Pseudomonas moraviensis; Stanleya pinnata; aerobic selenite reduction; elemental selenium nanoparticles; microchip capillary electrophoresis; multi-locus sequence analysis

PMID:
25968181
DOI:
10.1111/jam.12842
[Indexed for MEDLINE]
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