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Tumour Biol. 2015 Sep;36(10):8015-23. doi: 10.1007/s13277-015-3532-x. Epub 2015 May 14.

MicroRNA-218 inhibits bladder cancer cell proliferation, migration, and invasion by targeting BMI-1.

Author information

1
Department of Urology, The First Affiliated Hospital of Nanjing Medical University, 300, Guangzhou Road, Nanjing, Jiangsu, 210029, China.
2
Department of Urology, The First Affiliated Hospital of Nanjing Medical University, 300, Guangzhou Road, Nanjing, Jiangsu, 210029, China. doctorlvqiang@sina.com.

Abstract

MicroRNAs (miRNAs) are recognized as important molecules and have emerged as important gene regulators in tumorigenesis. Growing evidence suggested that miR-218 was a tumor suppressor in many human cancers. However, its underlying role in bladder cancer (BCa) remains unclear. The aim of this study was to explore the effect of miR-218 on the proliferation, migration, and invasion of BCa cells. We found that miR-218 was frequently downregulated in BCa tissues compared with normal adjacent tissues. In vitro and in vivo assays demonstrated that miR-218 overexpression in the BCa cells inhibited cell proliferation, migration, and invasion. Luciferase reporter assay showed that BMI-1 was a direct target of miR-218. In addition, we found that miR-218 regulated the expression of BMI-1 and its downstream target (PTEN) and participated in the phosphorylation of AKT. Our findings indicate that miR-218 functions as tumor suppressor in BCa, and the miR-218/BMI-1 axis may provide novel diagnostic and therapeutic strategies for the treatment of BCa.

KEYWORDS:

BMI-1; Bladder cancer; Invasion; MicroRNA-218; Migration; Proliferation

PMID:
25967457
DOI:
10.1007/s13277-015-3532-x
[Indexed for MEDLINE]

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