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Stem Cells. 2015 Aug;33(8):2400-15. doi: 10.1002/stem.2053. Epub 2015 May 27.

Mesenchymal Stem Cells Isolated From Human Gliomas Increase Proliferation and Maintain Stemness of Glioma Stem Cells Through the IL-6/gp130/STAT3 Pathway.

Author information

1
Department of Neurosurgery.
2
Brain Tumor Center, The University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA.
3
Department of Experimental Therapeutics.
4
Department of Radiation Oncology.
5
Department of Bioinformatics and Computational Biology.
6
Department of Cancer Biology, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.
7
Department of Leukemia.
8
Department of Neuro-Oncology, University of Utah Health Science Center, Salt Lake City, Utah, USA.

Abstract

Although mesenchymal stem cells (MSCs) have been implicated as stromal components of several cancers, their ultimate contribution to tumorigenesis and their potential to drive cancer stem cells, particularly in the unique microenvironment of human brain tumors, remain largely undefined. Consequently, using established criteria, we isolated glioma-associated-human MSCs (GA-hMSCs) from fresh human glioma surgical specimens for the first time. We show that these GA-hMSCs are nontumorigenic stromal cells that are phenotypically similar to prototypical bone marrow-MSCs. Low-passage genomic sequencing analyses comparing GA-hMSCs with matched tumor-initiating glioma stem cells (GSCs) suggest that most GA-hMSCs (60%) are normal cells recruited to the tumor (group 1 GA-hMSCs), although, rarely (10%), GA-hMSCs may differentiate directly from GSCs (group 2 GA-hMSCs) or display genetic patterns intermediate between these groups (group 3 GA-hMSCs). Importantly, GA-hMSCs increase proliferation and self-renewal of GSCs in vitro and enhance GSC tumorigenicity and mesenchymal features in vivo, confirming their functional significance within the GSC niche. These effects are mediated by GA-hMSC-secreted interleukin-6, which activates STAT3 in GSCs. Our results establish GA-hMSCs as a potentially new stromal component of gliomas that drives the aggressiveness of GSCs, and point to GA-hMSCs as a novel therapeutic target within gliomas.

KEYWORDS:

Brain tumor; Glioblastoma; Glioma stem cells; Interleukin-6; Mesenchymal stem cells; STAT3

PMID:
25966666
PMCID:
PMC4509942
DOI:
10.1002/stem.2053
[Indexed for MEDLINE]
Free PMC Article

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