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J Microbiol Methods. 2015 Aug;115:54-6. doi: 10.1016/j.mimet.2015.05.010. Epub 2015 May 9.

Assessment of four protocols for rapid bacterial identification from positive blood culture pellets by matrix-assisted laser desorption ionization-time of flight mass spectrometry (Vitek® MS).

Author information

1
Service de Bactériologie-Hygiène hospitalière, Nantes University Hospital, France.
2
Service de Bactériologie-Hygiène hospitalière, Nantes University Hospital, France; EA3826, Thérapeutiques cliniques et expérimentales des infections - Nantes Medicine school, France.
3
Service de biostatistique, Le Mans Hospital, France.
4
Service de Bactériologie-Hygiène hospitalière, Nantes University Hospital, France; EA3826, Thérapeutiques cliniques et expérimentales des infections - Nantes Medicine school, France. Electronic address: stephane.corvec@chu-nantes.fr.

Abstract

In this study, we developed and compared four protocols to prepare a bacterial pellet from 944 positive blood cultures for direct MALDI-TOF mass spectrometry Vitek® MS analysis. Protocol 4, tested on 200 monomicrobial samples, allowed 83% of bacterial identification. This easy, fast, cheap and accurate method is promising in daily practice, especially to limit broad range antibiotic treatment.

KEYWORDS:

Blood bottle culture; MALDI-TOF; Rapid identification

PMID:
25966643
DOI:
10.1016/j.mimet.2015.05.010
[Indexed for MEDLINE]

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