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Genet Mol Res. 2015 Apr 15;14(2):3450-8. doi: 10.4238/2015.April.15.8.

Effect of curcumin on p38MAPK expression in DSS-induced murine ulcerative colitis.

Author information

1
Department of Gastroenterology, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan Province, China ChangPingLicn@163.com.
2
West China School of Medicine, Sichuan University, Chengdu, Sichuan Province, China.
3
Department of Gastroenterology, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan Province, China.
4
Department of Gastroenterology, People's Hospital of Ya'an City, Sichuan Province, China.

Abstract

The aim of this study was to determine the therapeutic effect of curcumin on dextran sulfate sodium-induced ulcerative colitis (UC) and to explore the related mechanism. Sixty mice were randomly divided into 6 groups. A group was the normal control group; B group was the model group; C group was the 1.5 mg/kg dexamethasone group based on the B group; and D, E and F groups were 15, 30, and 60 mg/kg curcumin groups, respectively, based on the B group. The mice were killed 7 days after treatment; the expression of TNF-α and MPO in colon tissue was determined with ELISA, and colon p-p38MAPK and p38MAPK mRNA expression was evaluated by immunohistochemistry and RT-PCR, respectively. In the C, D, E, and F groups, TNF-α and MPO levels significantly decreased (P < 0.05), and the expression of p-p38MAPK also significantly decreased (P < 0.01). The expression of p38MAPK mRNA in the C, D, E, and F groups decreased (P < 0.01), and there was a statistically significant difference between the E and F groups (P < 0.01). Curcumin had a therapeutic effect, which probably played a role in UC treatment by inhibiting the p38MAPK signaling pathway, thereby reducing the release of TNF-α.

PMID:
25966111
DOI:
10.4238/2015.April.15.8
[Indexed for MEDLINE]
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