Format

Send to

Choose Destination
Muscle Nerve. 2016 Jan;53(1):91-5. doi: 10.1002/mus.24704.

Activity of Krebs cycle enzymes in mdx mice.

Author information

1
Laboratory of Experimental Neuroscience, Postgraduate Program in Health Sciences, University of Southern Santa Catarina, Palhoça, Santa Catarina, Brazil.
2
Laboratory of Neurosciences, Graduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, Criciúma, Santa Catarina, Brazil.
3
Laboratory of Bioenergetics, Postgraduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, 88806-000, Criciúma, Santa Catarina, Brazil.
4
Human Genome Research Center, Biosciences Institute, University of São Paulo, São Paulo, São Paulo, Brazil.
5
Center for Experimental Models in Psychiatry, Department of Psychiatry and Behavioral Sciences, University of Texas Medical School at Houston, Houston, Texas, USA.

Abstract

INTRODUCTION:

Duchenne muscular dystrophy (DMD) is a degenerative disease of skeletal, respiratory, and cardiac muscles caused by defects in the dystrophin gene. More recently, brain involvement has been verified. Mitochondrial dysfunction and oxidative stress may underlie the pathophysiology of DMD. In this study we evaluate Krebs cycle enzymes activity in the cerebral cortex, diaphragm, and quadriceps muscles of mdx mice.

METHODS:

Cortex, diaphragm, and quadriceps tissues from male dystrophic mdx and control mice were used.

RESULTS:

We observed increased malate dehydrogenase activity in the cortex; increased malate dehydrogenase and succinate dehydrogenase activities in the diaphragm; and increased citrate synthase, isocitrate dehydrogenase, and malate dehydrogenase activities in the quadriceps of mdx mice.

CONCLUSION:

This study showed increased activity of Krebs cycle enzymes in cortex, quadriceps, and diaphragm in mdx mice.

KEYWORDS:

Duchenne muscular dystrophy; Krebs cycle enzymes; cortex; diaphragm; mdx mice; quadriceps

PMID:
25965940
DOI:
10.1002/mus.24704
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center