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Anticancer Res. 2015 May;35(5):2691-8.

Demethoxycurcumin-induced DNA Damage Decreases DNA Repair-associated Protein Expression Levels in NCI-H460 Human Lung Cancer Cells.

Author information

1
Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C.
2
School of Pharmacy, China Medical University, Taichung, Taiwan, R.O.C.
3
Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C.
4
Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan, R.O.C.
5
Division of Chest Medicine, Department of Internal Medicine, Changhua Christian Hospital, Changhua, Taiwan, R.O.C. General Education Center, Chienkuo Technology University, Changhua, Taiwan, R.O.C.
6
Department of Surgery, China Medical University Hospital, Taichung, Taiwan, R.O.C.
7
Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C. Department of Internal Medicine, China Medical University Hospital, Taichung, Taiwan, R.O.C. jgchung@mail.cmu.edu.tw hsuwh@mail.cmuh.org.tw.
8
Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C. Department of Biotechnology, Asia University, Wu feng, Taichung, Taiwan, R.O.C. jgchung@mail.cmu.edu.tw hsuwh@mail.cmuh.org.tw.

Abstract

Demethoxycurcumin (DMC) is a key component of Chinese medicine (Turmeric) and has been proven effective in killing various cancer cells. Its role in inducing cytotoxic effects in many cancer cells has been reported, but its role regarding DNA damage on lung cancer cells has not been studied in detail. In the present study, we demonstrated DMC-induced DNA damage and condensation in NCI-H460 cells by using the Comet assay and DAPI staining examinations, respectively. Western blotting indicated that DMC suppressed the protein levels associated with DNA damage and repair, such as 14-3-3σ (an important checkpoint keeper of DNA damage response), DNA repair proteins breast cancer 1, early onset (BRCA1), O6-methylguanine-DNA methyltransferase (MGMT), mediator of DNA damage checkpoint 1 (MDC1), and p53 (tumor suppressor protein). DMC activated phosphorylated p53 and p-H2A.X (phospho Ser140) in NCI-H460 cells. Furthermore, we used confocal laser systems microscopy to examine the protein translocation. The results showed that DMC promotes the translocation of p-p53 and p-H2A.X from the cytosol to the nuclei in NCI-H460 cells. Taken together, DMC induced DNA damage and affected DNA repair proteins in NCI-H460 cells in vitro.

KEYWORDS:

DAPI staining; DNA damage; Demethoxycurcumin (DMC); comet assay; human lung cancer NCI-H460 cells

PMID:
25964547
[Indexed for MEDLINE]

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