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Comp Biochem Physiol B Biochem Mol Biol. 2015 Sep;187:55-61. doi: 10.1016/j.cbpb.2015.04.014. Epub 2015 May 9.

Arginine kinases from the marine feather star Tropiometra afra macrodiscus: The first finding of a prenylation signal sequence in metazoan phosphagen kinases.

Author information

1
Laboratory of Biochemistry, Faculty of Science, Kochi University, Kochi 780-8520, Japan.
2
Department of Zoology, National Museum of Nature and Science, Tsukuba 305-0005, Japan.
3
Faculty of Geo-environment Science, Rissho University, Magechi 1700, Kumagaya 360-0194, Japan.
4
Laboratory of Biochemistry, Faculty of Science, Kochi University, Kochi 780-8520, Japan. Electronic address: suzuki@kochi-u.ac.jp.

Abstract

Two arginine kinase cDNAs (AK1 and AK2) were isolated from the marine feather star Tropiometra afra macrodiscus, and the gene structure (exon/intron organization) of AK1 was determined. The cDNA-derived amino acid sequences and the exon/intron organization of the Tropiometra AK1 gene were homologous to those of a human creatine kinase (CK) as well as the AK of the sea cucumber Stichopus. Phylogenetic analysis also supports the close relationship between human CKs and echinoderm AKs, indicating that the latter AKs evolved from an ancestral CK gene. We observed that the Tropiometra AK1 gene has a novel C-terminal extension (approximately 50 amino acid residues) encoded by a unique exon. Moreover, a typical prenylation signal sequence (CSLL) was found at the C-terminal end of this extension, suggesting that AK1 is anchored to a membrane. AK2 had no such C-terminal extension. This is the first finding of a prenylation signal in metazoan phosphagen kinases. Recombinant Tropiometra AK1 and AK2 enzymes were successfully expressed in Escherichia coli, and their kinetic constants were determined. Both enzymes showed activity comparable to that of typical invertebrate AKs.

KEYWORDS:

Arginine kinase; Phosphagen kinase; Prenylation; Tropiometra afra

PMID:
25964010
DOI:
10.1016/j.cbpb.2015.04.014
[Indexed for MEDLINE]

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