Determination of pyruvic acid concentration using a bioluminescence system from Photobacterium leiognathi

Photochem Photobiol Sci. 2015 Jun;14(6):1163-8. doi: 10.1039/c5pp00118h.

Abstract

A novel, highly sensitive and selective bacterial luminescence method for the detection of pyruvic acid (PA) is reported here. This method is based on a reaction system catalyzed by lactate dehydrogenase (LDH) with the bacterial luciferase-FMN:NADH oxidoreductase bioluminescence system in vitro. The reduced nicotinamide adenine dinucleotide (NADH) involved in the LDH reaction system could be quantitatively analyzed by the bioluminescence system. A good linear relationship between the luminescence intensity and pyruvic acid concentration was exhibited within the range of 0.00014-0.001 mol l(-1), and the pyruvic acid detection limit was found to be 8.537 × 10(-5) mol l(-1). This method was successfully applied to the detection of PA in quail serum with a good recovery of over 70%.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Limit of Detection
  • Luciferases / chemistry
  • Luminescent Measurements / methods*
  • Photobacterium* / enzymology
  • Pyruvic Acid / chemistry*
  • Reproducibility of Results

Substances

  • Pyruvic Acid
  • Luciferases