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Antonie Van Leeuwenhoek. 2015 Jul;108(1):141-50. doi: 10.1007/s10482-015-0472-9. Epub 2015 May 10.

Two new species of the genus Micromonospora: Micromonospora palomenae sp. nov. and Micromonospora harpali sp. nov. isolated from the insects.

Author information

1
Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People's Republic of China.

Abstract

Two novel actinobacteria, strains NEAU-CX1(T) and NEAU-JC6(T), were isolated from nymphs of stinkbug (Palomena viridissima Poda) and a beetle (Harpalus sinicus Hope), respectively, collected from Harbin, Heilongjiang Province, China. A polyphasic study was carried out to establish the taxonomic positions of these strains. The phylogenetic analysis based on 16S rRNA gene sequence of strain NEAU-CX1(T) showed it to be most closely related to Micromonospora coxensis JCM 13248(T) (99.3 % sequence similarity), Micromonospora purpureochromogenes DSM 43821(T) (99.1 %) and Micromonospora halophytica JCM 3125(T) (98.6 %), and that of strain NEAU-JC6(T) to Micromonospora haikouensis DSM 45626(T) (99.3 %), Micromonospora carbonacea JCM 3139(T) (99.1 %) and Micromonospora krabiensis JCM 12869(T) (99.1 %). The phylogenetic analysis based on gyrB gene sequence of strain NEAU-CX1(T) showed it to be most closely related to M. purpureochromogenes DSM 43821(T) (98.0 % sequence similarity), and that of strain NEAU-JC6(T) to M. haikouensis DSM 45626(T) (99.0 %) and M. carbonacea JCM 3139(T) (98.0 %). A combination of DNA-DNA hybridization results and cultural and physiological properties indicated that the two strains can be distinguished from their closest phylogenetic relatives. Thus, strains NEAU-CX1(T) and NEAU-JC6(T) represent two novel species of the genus Micromonospora, for which the names Micromonospora palomenae sp. nov. and Micromonospora harpali sp. nov. are proposed. The type strains are NEAU-CX1(T) (=CGMCC 4.7175(T) = JCM 30056(T)) and NEAU-JC6(T) (=CGMCC 4.7173(T) = JCM 30055(T)).

PMID:
25957972
DOI:
10.1007/s10482-015-0472-9
[Indexed for MEDLINE]

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