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Biosens Bioelectron. 2015 Oct 15;72:31-6. doi: 10.1016/j.bios.2015.04.077. Epub 2015 Apr 24.

A rapid fluorescence "switch-on" assay for glutathione detection by using carbon dots-MnO2 nanocomposites.

Author information

1
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China.
2
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China; State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China.
3
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China; State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China. Electronic address: zhaohui.li@zzu.edu.cn.
4
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China; School of Chemistry & Chemical Engineering, Henan University of Technology, Zhengzhou 450001, PR China. Electronic address: qulingbo@zzu.edu.cn.

Abstract

Glutathione (GSH) serves many cellular functions and plays crucial roles in human pathologies. Simple and sensitive sensors capable of detecting GSH would be useful tools to understand the mechanism of diseases. In this work, a rapid fluorescence "switch-on" assay was developed to detect trace amount of GSH based on carbon dots-MnO2 nanocomposites, which was fabricated through in situ synthesis of MnO2 nanosheets in carbon dots colloid solution. Due to the formation of carbon dots-MnO2 nanocomposites, fluorescence of carbon dots could be quenched efficiently by MnO2 nanosheeets through fluorescence resonance energy transfer (FRET). However, the presence of GSH would reduce MnO2 nanosheets to Mn(2+) ions and subsequently release carbon dots, which resulted in sufficient recovery of fluorescent signal. This proposed assay demonstrated highly selectivity toward GSH with a detection limit of 300nM. Moreover, this method has also shown sensitive responses to GSH in human serum samples, which indicated its great potential to be used in disease diagnosis. As no requirement of any further functionalization of these as-prepared nanomaterials, this sensing system shows remarkable advantages including very fast and simple, cost-effective as well as environmental-friendly, which suggest that this new strategy could serve as an efficient tool for analyzing GSH level in biosamples.

KEYWORDS:

Carbon dots; Fluorescnece detection; Glutathione; MnO(2) nanosheets; Nanocomposites

PMID:
25957074
DOI:
10.1016/j.bios.2015.04.077
[Indexed for MEDLINE]

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