Lipopolysaccharide pretreatment inhibits LPS-induced human umbilical cord mesenchymal stem cell apoptosis via upregulating the expression of cellular FLICE-inhibitory protein

Yu Sen Hou; Ling Ying Liu; Jia Ke Chai; Yong Hui Yu; Hong Jie Duan; Quan Hu; Hui Nan Yin; Yi He Wang; Shu Bo Zhuang; Jun Fan; Wan Li Chu; Li Ma

doi:10.3892/mmr.2015.3723

Lipopolysaccharide pretreatment inhibits LPS-induced human umbilical cord mesenchymal stem cell apoptosis via upregulating the expression of cellular FLICE-inhibitory protein

Mol Med Rep. 2015 Aug;12(2):2521-8. doi: 10.3892/mmr.2015.3723. Epub 2015 May 4.

Authors

Yu Sen Hou¹, Ling Ying Liu¹, Jia Ke Chai¹, Yong Hui Yu¹, Hong Jie Duan¹, Quan Hu¹, Hui Nan Yin¹, Yi He Wang¹, Shu Bo Zhuang¹, Jun Fan¹, Wan Li Chu¹, Li Ma¹

Affiliation

¹ Department of Burns and Plastic Surgery, The First Affiliated Hospital of PLA General Hospital, Beijing 100048, P.R. China.

Abstract

Mesenchymal stem cell (MSC)-based regenerative therapy is currently regarded as a novel approach with which to repair damaged tissues. However, the efficiency of MSC transplantation is limited due to the low survival rate of engrafted MSCs. Lipopolysaccharide (LPS) production is increased in numerous diseases and serves an essential function in the regulation of apoptosis in a variety of cell types. Previous studies have indicated that low-dose LPS pretreatment contributes to cytoprotection. In the current study, LPS was demonstrated to induce apoptosis in human umbilical cord mesenchymal stem cells (hUCMSCs) via the activation of caspase, in a dose-dependent manner. Low-dose LPS pretreatment may protect hUCMSCs against apoptosis induced by high-dose LPS, by upregulating the expression of cellular FADD-like IL-1β-converting enzyme-inhibitory protein (c-FLIP). The results of the present study indicate that pretreatment with an appropriate concentration of LPS may alleviate high-dose LPS-induced apoptosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis / drug effects
CASP8 and FADD-Like Apoptosis Regulating Protein / agonists
CASP8 and FADD-Like Apoptosis Regulating Protein / antagonists & inhibitors
CASP8 and FADD-Like Apoptosis Regulating Protein / genetics
CASP8 and FADD-Like Apoptosis Regulating Protein / immunology*
Caspase 3 / genetics
Caspase 3 / immunology
Caspase 8 / genetics
Caspase 8 / immunology
Cell Survival / drug effects
Cytoprotection
Fetal Blood / cytology
Fetal Blood / drug effects
Fetal Blood / immunology
Gene Expression Regulation
Hormesis / immunology*
Humans
Lipopolysaccharides / pharmacology*
Mesenchymal Stem Cells / cytology
Mesenchymal Stem Cells / drug effects*
Mesenchymal Stem Cells / immunology
Primary Cell Culture
RNA, Small Interfering / genetics
RNA, Small Interfering / immunology
Signal Transduction

Substances

CASP8 and FADD-Like Apoptosis Regulating Protein
CFLAR protein, human
Lipopolysaccharides
RNA, Small Interfering
CASP8 protein, human
Caspase 3
Caspase 8