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Elife. 2015 May 7;4. doi: 10.7554/eLife.07118.

Coordination of peptidoglycan synthesis and outer membrane constriction during Escherichia coli cell division.

Author information

1
Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, United States.
2
Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle Upon Tyne, United Kingdom.
3
Membrane Biochemistry and Biophysics, Bijvoet Centre for Biomolecular Research, University of Utrecht, Utrecht, The Netherlands.
4
Bacterial Cell Biology, Swammerdam Institute for Life Sciences, Faculty of Science, University of Amsterdam, Amsterdam, The Netherlands.
5
Biophysics Program, Stanford University, Stanford, United States.
6
Genome Biology Unit, European Molecular Biology Laboratory Heidelberg, Heidelberg, Germany.
7
Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Utrecht, The Netherlands.
8
Institut de Biologie Structurale, Université Grenoble Alpes, Grenoble, France.

Abstract

To maintain cellular structure and integrity during division, Gram-negative bacteria must carefully coordinate constriction of a tripartite cell envelope of inner membrane, peptidoglycan (PG), and outer membrane (OM). It has remained enigmatic how this is accomplished. Here, we show that envelope machines facilitating septal PG synthesis (PBP1B-LpoB complex) and OM constriction (Tol system) are physically and functionally coordinated via YbgF, renamed CpoB (Coordinator of PG synthesis and OM constriction, associated with PBP1B). CpoB localizes to the septum concurrent with PBP1B-LpoB and Tol at the onset of constriction, interacts with both complexes, and regulates PBP1B activity in response to Tol energy state. This coordination links PG synthesis with OM invagination and imparts a unique mode of bifunctional PG synthase regulation by selectively modulating PBP1B cross-linking activity. Coordination of the PBP1B and Tol machines by CpoB contributes to effective PBP1B function in vivo and maintenance of cell envelope integrity during division.

KEYWORDS:

E. coli; cell division; cell envelope; infectious disease; microbiology; outer membrane; peptidoglycan

PMID:
25951518
PMCID:
PMC4458516
DOI:
10.7554/eLife.07118
[Indexed for MEDLINE]
Free PMC Article

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