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Methods Enzymol. 2015;557:329-48. doi: 10.1016/bs.mie.2014.12.005. Epub 2015 Mar 17.

Solution NMR Structure Determination of Polytopic α-Helical Membrane Proteins: A Guide to Spin Label Paramagnetic Relaxation Enhancement Restraints.

Author information

1
Department of Chemistry, University of Virginia, Charlottesville, Virginia, USA. Electronic address: lcolumbus@gmail.com.
2
Department of Biochemistry and Center for Structural Biology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.

Abstract

Solution nuclear magnetic resonance structures of polytopic α-helical membrane proteins require additional restraints beyond the traditional Nuclear Overhauser Effect (NOE) restraints. Several methods have been developed and this review focuses on paramagnetic relaxation enhancement (PRE). Important aspects of spin labeling, PRE measurements, structure calculations, and structural quality are discussed.

KEYWORDS:

Membrane proteins; Nitroxide spin label; Nuclear magnetic resonance; Paramagnetic relaxation enhancement; Polytopic; Site-directed spin labeling; α-Helical

PMID:
25950972
DOI:
10.1016/bs.mie.2014.12.005
[Indexed for MEDLINE]

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