Electrochemiluminescence immunosensor based on multifunctional luminol-capped AuNPs@Fe3O4 nanocomposite for the detection of mucin-1

Biosens Bioelectron. 2015 Sep 15:71:407-413. doi: 10.1016/j.bios.2015.04.062. Epub 2015 Apr 23.

Abstract

In this work, a novel and multifunctional nanocomposite of luminol capped gold modified Fe3O4 (Lu-AuNPs@Fe3O4) was utilized as the carrier of secondary antibody (Ab2) to fabricate a sandwiched electrochemiluminescence (ECL) immunosensor for ultrasensitive detection of mucin-1 (MUC1). Herein, the luminol capped gold nanoparticles (Lu-AuNPs) were synthesized with HAuCl4 and luminol by the help of NaBH4 at room temperature, and then Lu-AuNPs were adsorbed on the Fe3O4 magnetic nanoparticles (MNPs) to form the nanocomposite of Lu-AuNPs@Fe3O4 via electrostatic interaction. Fe3O4 MNPs in Lu-AuNPs@Fe3O4 exhibited excellent conductivity and admirable catalytic activity in H2O2 decomposition, which could enhance the ECL efficiency of luminol-H2O2 system. In addition, the substrates of gold coated ZnO nanoparticles (AuNPs@ZnO), providing large specific surface areas for primary antibody (Ab1) capturing, were modified on the electrode. As a result, a wide linear range of 7 orders of magnitude from 10 fg/mL to 10 ng/mL was obtained with an ultralow detection limit of 4.5 fg/mL for MUC1.

Keywords: Electrochemiluminescence; Immunosensor; Luminol; Lu–AuNPs@Fe(3)O(4); MUC1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Conductometry / instrumentation*
  • Equipment Design
  • Equipment Failure Analysis
  • Gold / chemistry
  • Immunoassay / instrumentation*
  • Luminescent Measurements / instrumentation*
  • Luminol / chemistry*
  • Magnetite Nanoparticles / chemistry*
  • Metal Nanoparticles / chemistry
  • Mucin-1 / immunology
  • Nanocomposites / chemistry*
  • Nanocomposites / ultrastructure
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Magnetite Nanoparticles
  • Mucin-1
  • Luminol
  • Gold