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PLoS One. 2015 May 6;10(5):e0125354. doi: 10.1371/journal.pone.0125354. eCollection 2015.

Generation and Imaging of Transgenic Mice that Express G-CaMP7 under a Tetracycline Response Element.

Author information

1
RIKEN Brain Science Institute, Wako, Saitama, Japan; PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama, Japan.
2
RIKEN Brain Science Institute, Wako, Saitama, Japan.
3
Brain Science Institute, Saitama University, Saitama, Japan.
4
RIKEN Brain Science Institute, Wako, Saitama, Japan; Brain Science Institute, Saitama University, Saitama, Japan.

Abstract

The spatiotemporally controlled expression of G-CaMP fluorescent calcium indicator proteins can facilitate reliable imaging of brain circuit activity. Here, we generated a transgenic mouse line that expresses G-CaMP7 under a tetracycline response element. When crossed with a forebrain-specific tetracycline-controlled transactivator driver line, the mice expressed G-CaMP7 in defined cell populations in a tetracycline-controlled manner, notably in pyramidal neurons in layer 2/3 of the cortex and in the CA1 area of the hippocampus; this expression allowed for imaging of the in vivo activity of these circuits. This mouse line thus provides a useful genetic tool for controlled G-CaMP expression in vivo.

PMID:
25946002
PMCID:
PMC4422725
DOI:
10.1371/journal.pone.0125354
[Indexed for MEDLINE]
Free PMC Article

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