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Nature. 2015 May 21;521(7552):316-21. doi: 10.1038/nature14413. Epub 2015 May 6.

An alternative pluripotent state confers interspecies chimaeric competency.

Author information

1
The Salk Institute for Biological Studies, Gene Expression Laboratory, La Jolla, California 92037, USA.
2
1] Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, California 92037, USA [2] The Salk Institute for Biological Studies, Genomic Analysis Laboratory, La Jolla, California 92037, USA.
3
The Salk Institute for Biological Studies, Genomic Analysis Laboratory, La Jolla, California 92037, USA.
4
The Salk Institute for Biological Studies, Integrated Genomics, La Jolla, California 92037, USA.
5
Ludwig Institute for Cancer Research, University of California, San Diego School of Medicine, Department of Cellular and Molecular Medicine, 9500 Gilman Drive, La Jolla, California 92093-0653, USA.
6
1] The Salk Institute for Biological Studies, Gene Expression Laboratory, La Jolla, California 92037, USA [2] Life Science Center, Tsukuba Advanced Research Alliance, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8577, Japan.
7
Grado en Medicina, Universidad Católica, San Antonio de Murcia, Campus de los Jerónimos, 135, Guadalupe 30107, Spain.
8
1] Grado en Medicina, Universidad Católica, San Antonio de Murcia, Campus de los Jerónimos, 135, Guadalupe 30107, Spain [2] Fundacion Pedro Guillen, Clínica Cemtro, Avenida Ventisquero de la Condesa, 42, 28035 Madrid, Spain.
9
Hospital Clinic of Barcelona, Carrer Villarroel, 170, 08036 Barcelona, Spain.
10
University of California, Davis, Davis, California 95616, USA.
11
The Salk Institute for Biological Studies, Peptide Biology Laboratory, La Jolla, California 92037, USA.

Abstract

Pluripotency, the ability to generate any cell type of the body, is an evanescent attribute of embryonic cells. Transitory pluripotent cells can be captured at different time points during embryogenesis and maintained as embryonic stem cells or epiblast stem cells in culture. Since ontogenesis is a dynamic process in both space and time, it seems counterintuitive that these two temporal states represent the full spectrum of organismal pluripotency. Here we show that by modulating culture parameters, a stem-cell type with unique spatial characteristics and distinct molecular and functional features, designated as region-selective pluripotent stem cells (rsPSCs), can be efficiently obtained from mouse embryos and primate pluripotent stem cells, including humans. The ease of culturing and editing the genome of human rsPSCs offers advantages for regenerative medicine applications. The unique ability of human rsPSCs to generate post-implantation interspecies chimaeric embryos may facilitate our understanding of early human development and evolution.

PMID:
25945737
PMCID:
PMC5278765
DOI:
10.1038/nature14413
[Indexed for MEDLINE]
Free PMC Article

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