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Iran J Basic Med Sci. 2015 Mar;18(3):301-6.

Aqueous extract of Zizyphus jujuba fruit attenuates glucose induced neurotoxicity in an in vitro model of diabetic neuropathy.

Author information

1
Department of Physiology, School of Veterinary Medicine, Lorestan University, Khorramabad, Iran.
2
Kerman Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran.
3
Young Researchers Club, Khoramabad Brach, Islamic Azad University, Khoramabad, Iran.

Abstract

OBJECTIVES:

The neuroprotective effect of fruit aqueous extract of Ziziphus jujuba Lam on glucose-induced neurotoxicity in PC12 cells as an appropriate in vitro model of diabetic neuropathy was investigated.

MATERIALS AND METHODS:

Cell viability was determined by the MTT assay. Cellular reactive oxygen species (ROS) generation was measured by DCFH-DA analysis. Cleaved caspase-3, a biochemical parameter of cellular apoptosis, was measured by western blot analysis.

RESULTS:

Our data showed that a 4-fold elevation in glucose levels within the medium significantly reduced cell viability, increased intracellular ROS and caspase-3 activation in PC12 cells after 24 hr. Incubation of the high glucose medium cells with 300-μg/ml Z. jujuba fruit (ZJF) extract decreased the high glucose-induced cell toxicity and prevented caspase-3 activation and excited ROS generation.

CONCLUSION:

Thus, we concluded that the aqueous extract of Z. jujuba protects against hyperglycaemia-induced cellular toxicity. This could be associated with the prevention of ROS generation and neural apoptosis. Moreover, the results suggest that the ZJF has a therapeutic potential to attenuate diabetes complications such as neuropathy.

KEYWORDS:

Apoptosis; Diabetes; Neuropathy; Ziziphus jujube

PMID:
25945244
PMCID:
PMC4414997

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