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FEBS Lett. 2015 Jun 4;589(13):1467-75. doi: 10.1016/j.febslet.2015.04.039. Epub 2015 May 1.

Similar regulation of two distinct UL24 promoters by regulatory proteins of equine herpesvirus type 1 (EHV-1).

Author information

1
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
2
College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China.
3
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China; College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China. Electronic address: xjw@hvri.ac.cn.

Abstract

To characterise the pattern of the transcriptional regulation of equine herpesvirus type 1 (EHV-1) UL24 by regulatory proteins, we identified two distinct promoter regions and two transcription initiation (Tci) sites located upstream of the UL24 open reading frame (ORF). The ORF proximal promoter exhibited higher cis-activity than that of the distal one. Contrary to the former, the latter performed its function dependent on an initiator (INR) due to its lack of a TATA box. Our results showed that the EHV-1 regulatory proteins EICP0, EICP22 and ETIF trans-activated the two promoters, whereas IEP and IR2P displayed negative regulation. In summary, the regulatory proteins exhibited similar regulatory patterns for the two distinct promoters of EHV-1 UL24.

KEYWORDS:

Double promoters; Equine herpesvirus type 1; Regulatory protein; Transcriptional regulation; UL24

PMID:
25937123
DOI:
10.1016/j.febslet.2015.04.039
[Indexed for MEDLINE]
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