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Neuropsychopharmacology. 2015 Nov;40(12):2753-63. doi: 10.1038/npp.2015.125. Epub 2015 May 4.

Increased GABAergic Efficacy of Central Amygdala Projections to Neuropeptide S Neurons in the Brainstem During Fear Memory Retrieval.

Author information

1
Institute of Physiology I, Westfälische Wilhelms-Universität Münster, Münster, Germany.
2
Department of Neurology, Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB), Universitätsmedizin Göttingen, Göttingen, Germany.

Abstract

The canonical view on the central amygdala has evolved from a simple output station towards a highly organized microcircuitry, in which types of GABAergic neurons in centrolateral (CeL) and centromedial (CeM) subnuclei regulate fear expression and generalization. How these specific neuronal populations are connected to extra-amygdaloid target regions remains largely unknown. Here we show in mice that a subpopulation of GABAergic CeL and CeM neurons projects monosynaptically to brainstem neurons expressing neuropeptide S (NPS). The CeL neurons are PKCδ-negative and are activated during conditioned fear. During fear memory retrieval, the efficacy of this GABAergic influence on NPS neurons is enhanced. Moreover, a large proportion of these neurons (~50%) contain prodynorphin and somatostatin, two neuropeptides inhibiting NPS neurons. We conclude that CeL and CeM neurons inhibit NPS neurons in the brainstem by GABA release and that efficacy of this connection is strengthened upon fear memory retrieval. Thereby, this pathway provides a possible feedback mechanism between amygdala and brainstem routes involved in fear and stress coping.

PMID:
25936641
PMCID:
PMC4864651
DOI:
10.1038/npp.2015.125
[Indexed for MEDLINE]
Free PMC Article

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