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Fertil Steril. 2015 Jul;104(1):217-24.e2. doi: 10.1016/j.fertnstert.2015.03.023. Epub 2015 Apr 29.

Drospirenone induces decidualization in human eutopic endometrial stromal cells and reduces DNA synthesis of human endometriotic stromal cells.

Author information

1
Department of Obstetrics and Gynecology, University of Tokyo, Tokyo, Japan.
2
Department of Obstetrics and Gynecology, University of Tokyo, Tokyo, Japan. Electronic address: kawotan-tky@umin.ac.jp.

Abstract

OBJECTIVE:

To investigate the in vitro effect of drospirenone on human eutopic endometrial (EuSC) and ectopic endometriotic stromal cells (EcSC).

DESIGN:

Comparative and laboratory study. The experimental procedures were approved by the Institutional Review Board of the University of Tokyo (registration no. 0324-4).

SETTING:

University research laboratory.

PATIENTS(S):

Eight patients undergoing hysterectomy for benign gynecologic disease and 19 patients undergoing cystectomy or adnectomy for endometriosis.

INTERVENTION(S):

EuSC and EcSC were treated with drospirenone.

MAIN OUTCOME MEASURE(S):

For the analysis of decidualization of EuSC, cells were observed using microscopy, and the production of PRL was measured using enzyme immunoassay. For the analysis of DNA synthesis of EcSC, 5-bromo-2'-deoxyuridine incorporation was measured by ELISA. Cells in apoptosis were detected and measured by flow cytometry.

RESULT(S):

Drospirenone induced decidualization in EuSC, and the induction was negated by RU486. Drospirenone reduced DNA synthesis on EcSC, and this reduction was negated by RU486 or P receptor silencing, but not by aldosterone or mineralocorticoid receptor silencing. Drospirenone did not cause EcSC to undergo apoptosis.

CONCLUSION(S):

Our study demonstrates the direct effects of drospirenone: decidualization of EuSC and reduced DNA synthesis of EcSC, but it does not cause EsSC apoptosis.

KEYWORDS:

Drospirenone; decidualization; endometriosis; endometrium; oral contraceptive(s)

[Indexed for MEDLINE]

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