Format

Send to

Choose Destination
PLoS One. 2015 May 1;10(5):e0124629. doi: 10.1371/journal.pone.0124629. eCollection 2015.

Reed-Sternberg cells form by abscission failure in the presence of functional Aurora B kinase.

Author information

1
Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.
2
Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal; Cell Division Unit, Department of Experimental Biology, Faculdade de Medicina, Universidade do Porto, Alameda Prof. Hernâni Monteiro, Porto, Portugal.
3
The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California San Francsicso, San Francisco, United States of America.
4
Instituto de Patologia e Imunologia Molecular da Universidade do Porto, IPATIMUP, Porto, Portugal.
5
MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, United Kingdom.
6
Wellcome Trust Centre for Cell Biology, University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh, United Kingdom.

Abstract

Large multinucleated Reed-Sternberg cells (RS) and large mononucleated Hodgkin cells (H) are traditionally considered to be the neoplastic population in classical Hodgkin lymphoma, (cHL) and postulated to promote the disease. However, the contribution of these larger cells to the progression of cHL remains debatable. We used established cHL cell lines and cHL cellular fractions composed of small mononucleated cells only or enriched in large RS/H cells to investigate RS/H cell origin and to characterize the cells which they derive from. We confirm that the small mononucleated cells give rise to RS/H cells, and we show that the latter proliferate significantly more slowly than the small cells. By using live-cell imaging, we demonstrate that binucleated RS cells are generated by failure of abscission when a few small cells attempt to divide. Finally, our results reveal that the small mononucleated cells are chromosomally unstable, but this is unlikely to be related to a malfunctioning chromosomal passenger protein complex. We propose that the small mononucleated cells, rather than the RS/H cells, are the main drivers of cHL.

PMID:
25933052
PMCID:
PMC4416800
DOI:
10.1371/journal.pone.0124629
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center