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J Korean Med Sci. 2015 May;30(5):576-85. doi: 10.3346/jkms.2015.30.5.576. Epub 2015 Apr 15.

The Effect of Umbilical Cord Blood Derived Mesenchymal Stem Cells in Monocrotaline-induced Pulmonary Artery Hypertension Rats.

Author information

1
Department of Pediatrics, Ewha Womans University School of Medicine, Seoul, Korea.
2
Department of Thoracic and Cardiovascular Surgery, Ewha Womans University School of Medicine, Seoul, Korea.
3
Department of Pathology, Ewha Womans University School of Medicine, Seoul, Korea.
4
Biomedical Research Institute, MEDIPOST, Co., Seoul, Korea.
5
Division of Integrative Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Korea.
6
Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang, Korea.

Abstract

Pulmonary arterial hypertension (PAH) causes right ventricular failure due to a gradual increase in pulmonary vascular resistance. The purposes of this study were to confirm the engraftment of human umbilical cord blood-mesenchymal stem cells (hUCB-MSCs) placed in the correct place in the lung and research on changes of hemodynamics, pulmonary pathology, immunomodulation and several gene expressions in monocrotaline (MCT)-induced PAH rat models after hUCB-MSCs transfusion. The rats were grouped as follows: the control (C) group; the M group (MCT 60 mg/kg); the U group (hUCB-MSCs transfusion). They received transfusions via the external jugular vein a week after MCT injection. The mean right ventricular pressure (RVP) was significantly reduced in the U group after the 2 week. The indicators of RV hypertrophy were significantly reduced in the U group at week 4. Reduced medial wall thickness in the pulmonary arteriole was noted in the U group at week 4. Reduced number of intra-acinar muscular pulmonary arteries was observed in the U group after 2 week. Protein expressions such as endothelin (ET)-1, endothelin receptor A (ERA), endothelial nitric oxide synthase (eNOS) and matrix metalloproteinase (MMP)-2 significantly decreased at week 4. The decreased levels of ERA, eNOS and MMP-2 immunoreactivity were noted by immnohistochemical staining. After hUCB-MSCs were administered, there were the improvement of RVH and mean RVP. Reductions in several protein expressions and immunomodulation were also detected. It is suggested that hUCB-MSCs may be a promising therapeutic option for PAH.

KEYWORDS:

Cord Blood Stem Cell Transfusion; Gene Expression; Hypertension; Monocrotaline; Pulmonary

PMID:
25931788
PMCID:
PMC4414641
DOI:
10.3346/jkms.2015.30.5.576
[Indexed for MEDLINE]
Free PMC Article

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