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Stem Cell Res Ther. 2014 Sep 26;5(4):100. doi: 10.1186/scrt500.

Aromatic-turmerone induces neural stem cell proliferation in vitro and in vivo.

Author information

1
Cognitive Neuroscience, Institute of Neuroscience and Medicine (INM-3), Research Centre Juelich, Leo-Brandt-Straße 52425, Jülich, Germany. joerg@germanynet.de.
2
Department of Neurology, University Hospital of Cologne, Cologne, Germany. joerg@germanynet.de.
3
Department of Neurology, University Hospital of Cologne, Cologne, Germany. rebecca.klein@uk-koeln.de.
4
Max Planck Institute for Neurological Research, Cologne, Germany. rebecca.klein@uk-koeln.de.
5
Max Planck Institute for Neurological Research, Cologne, Germany. bernd.neumaier@nf.mpg.de.
6
Max Planck Institute for Neurological Research, Cologne, Germany. rudolf.graf@nf.mpg.de.
7
Cognitive Neuroscience, Institute of Neuroscience and Medicine (INM-3), Research Centre Juelich, Leo-Brandt-Straße 52425, Jülich, Germany. gereon.fink@uk-koeln.de.
8
Department of Neurology, University Hospital of Cologne, Cologne, Germany. gereon.fink@uk-koeln.de.
9
Cognitive Neuroscience, Institute of Neuroscience and Medicine (INM-3), Research Centre Juelich, Leo-Brandt-Straße 52425, Jülich, Germany. michael.schroeter@uk-koeln.de.
10
Department of Neurology, University Hospital of Cologne, Cologne, Germany. michael.schroeter@uk-koeln.de.
11
Max Planck Institute for Neurological Research, Cologne, Germany. michael.schroeter@uk-koeln.de.
12
Cognitive Neuroscience, Institute of Neuroscience and Medicine (INM-3), Research Centre Juelich, Leo-Brandt-Straße 52425, Jülich, Germany. adele.rueger@uk-koeln.de.
13
Department of Neurology, University Hospital of Cologne, Cologne, Germany. adele.rueger@uk-koeln.de.
14
Max Planck Institute for Neurological Research, Cologne, Germany. adele.rueger@uk-koeln.de.

Abstract

INTRODUCTION:

Aromatic (ar-) turmerone is a major bioactive compound of the herb Curcuma longa. It has been suggested that ar-turmerone inhibits microglia activation, a property that may be useful in treating neurodegenerative disease. Furthermore, the effects of ar-turmerone on neural stem cells (NSCs) remain to be investigated.

METHODS:

We exposed primary fetal rat NSCs to various concentrations of ar-turmerone. Thereafter, cell proliferation and differentiation potential were assessed. In vivo, naïve rats were treated with a single intracerebroventricular (i.c.v.) injection of ar-turmerone. Proliferative activity of endogenous NSCs was assessed in vivo, by using noninvasive positron emission tomography (PET) imaging and the tracer [(18)F]-fluoro-L-thymidine ([(18)F]FLT), as well as ex vivo.

RESULTS:

In vitro, ar-turmerone increased dose-dependently the number of cultured NSCs, because of an increase in NSC proliferation (P < 0.01). Proliferation data were supported by qPCR-data for Ki-67 mRNA. In vitro as well as in vivo, ar-turmerone promoted neuronal differentiation of NSCs. In vivo, after i.c.v. injection of ar-turmerone, proliferating NSCs were mobilized from the subventricular zone (SVZ) and the hippocampus of adult rats, as demonstrated by both [(18)F]FLT-PET and histology (P < 0.05).

CONCLUSIONS:

Both in vitro and in vivo data suggest that ar-turmerone induces NSC proliferation. Ar-turmerone thus constitutes a promising candidate to support regeneration in neurologic disease.

PMID:
25928248
PMCID:
PMC4180255
DOI:
10.1186/scrt500
[Indexed for MEDLINE]
Free PMC Article

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