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PLoS One. 2015 Apr 30;10(4):e0125046. doi: 10.1371/journal.pone.0125046. eCollection 2015.

Phytohormonal networks promote differentiation of fiber initials on pre-anthesis cotton ovules grown in vitro and in planta.

Author information

1
Cotton Fiber Bioscience Research Unit, USDA-ARS-SRRC, New Orleans, Louisiana, United States of America.
2
Institute for Cellular and Molecular Biology and Center for Computational Biology and Bioinformatics, The University of Texas at Austin, Austin, Texas, United States of America.
3
Department of Soil and Crop Science, Texas A&M University, College Station, Texas, United States of America.
4
Plains Area, USDA-ARS, Fort Collins, Colorado, United States of America.
5
Crop Genetics Research Unit, USDA-ARS, Stoneville, Mississippi, United States of America.

Abstract

The number of cotton (Gossypium sp.) ovule epidermal cells differentiating into fiber initials is an important factor affecting cotton yield and fiber quality. Despite extensive efforts in determining the molecular mechanisms regulating fiber initial differentiation, only a few genes responsible for fiber initial differentiation have been discovered. To identify putative genes directly involved in the fiber initiation process, we used a cotton ovule culture technique that controls the timing of fiber initial differentiation by exogenous phytohormone application in combination with comparative expression analyses between wild type and three fiberless mutants. The addition of exogenous auxin and gibberellins to pre-anthesis wild type ovules that did not have visible fiber initials increased the expression of genes affecting auxin, ethylene, ABA and jasmonic acid signaling pathways within 1 h after treatment. Most transcripts expressed differentially by the phytohormone treatment in vitro were also differentially expressed in the ovules of wild type and fiberless mutants that were grown in planta. In addition to MYB25-like, a gene that was previously shown to be associated with the differentiation of fiber initials, several other differentially expressed genes, including auxin/indole-3-acetic acid (AUX/IAA) involved in auxin signaling, ACC oxidase involved in ethylene biosynthesis, and abscisic acid (ABA) 8'-hydroxylase an enzyme that controls the rate of ABA catabolism, were co-regulated in the pre-anthesis ovules of both wild type and fiberless mutants. These results support the hypothesis that phytohormonal signaling networks regulate the temporal expression of genes responsible for differentiation of cotton fiber initials in vitro and in planta.

PMID:
25927364
PMCID:
PMC4415818
DOI:
10.1371/journal.pone.0125046
[Indexed for MEDLINE]
Free PMC Article

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