Abstract
Behcet's disease (BD) is a recurrent pathema with a typical symptom of inflammation involved in many organs. Previous report indicated that the serum of Korean patients with BD stimulates membrane expression of hnRNP A2/B1 in endothelial cells. In this study, the target 35 kDa recombinant human hnRNP A2/B1 were over-expressed and purified, then sequenced with MALDI-TOF- TOF mass spectrometry. Western blotting and ELISA were applied to detect serum reactivity against hnRNP A2/B1 respectively. The results demonstrate that hnRNP A2/B1 is an autoantigen of BD in Han Chinese population.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Asian People
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Autoantibodies / blood*
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Autoantigens / genetics
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Autoantigens / immunology*
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Autoantigens / isolation & purification
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Autoantigens / metabolism
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Behcet Syndrome / immunology*
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China
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Cloning, Molecular
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Enzyme-Linked Immunosorbent Assay
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Heterogeneous-Nuclear Ribonucleoprotein Group A-B / genetics
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Heterogeneous-Nuclear Ribonucleoprotein Group A-B / immunology*
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Heterogeneous-Nuclear Ribonucleoprotein Group A-B / isolation & purification
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Heterogeneous-Nuclear Ribonucleoprotein Group A-B / metabolism
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Humans
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Plasmids / genetics
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology*
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
Substances
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Autoantibodies
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Autoantigens
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Heterogeneous-Nuclear Ribonucleoprotein Group A-B
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Recombinant Proteins