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Elife. 2015 Apr 29;4. doi: 10.7554/eLife.06496.

The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin.

Author information

1
Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Champaign, United States.
2
Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Champaign, United States.
3
Université Paris Diderot, Sorbonne Paris Cité, Laboratoire Epigénétique et Destin Cellulaire, UMR7216, Centre National de la Recherche Scientifique, Paris, France.

Abstract

Heterochromatic domains are enriched with repressive histone marks, including histone H3 lysine 9 methylation, written by lysine methyltransferases (KMTs). The pre-replication complex protein, origin recognition complex-associated (ORCA/LRWD1), preferentially localizes to heterochromatic regions in post-replicated cells. Its role in heterochromatin organization remained elusive. ORCA recognizes methylated H3K9 marks and interacts with repressive KMTs, including G9a/GLP and Suv39H1 in a chromatin context-dependent manner. Single-molecule pull-down assays demonstrate that ORCA-ORC (Origin Recognition Complex) and multiple H3K9 KMTs exist in a single complex and that ORCA stabilizes H3K9 KMT complex. Cells lacking ORCA show alterations in chromatin architecture, with significantly reduced H3K9 di- and tri-methylation at specific chromatin sites. Changes in heterochromatin structure due to loss of ORCA affect replication timing, preferentially at the late-replicating regions. We demonstrate that ORCA acts as a scaffold for the establishment of H3K9 KMT complex and its association and activity at specific chromatin sites is crucial for the organization of heterochromatin structure.

KEYWORDS:

ORCA/LRWD1; cell biology; chromosomes; genes; heterochromatin; human; lysine methyl transferase; replication; single-molecule pull down

PMID:
25922909
PMCID:
PMC4442312
DOI:
10.7554/eLife.06496
[Indexed for MEDLINE]
Free PMC Article

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