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Int J Syst Evol Microbiol. 2015 Aug;65(8):2491-5. doi: 10.1099/ijs.0.000289. Epub 2015 Apr 28.

Thermus amyloliquefaciens sp. nov., isolated from a hot spring sediment sample.

Author information

1
1​ Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China 2​ State Key Laboratory of Microbial Metabolism and State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, Shanghai, 201100, PR China.
2
1​ Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China 3​ College of Life Sciences and Technology, Xinxiang Medical University, Xinxiang, 453003, PR China.
3
1​ Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China.
4
1​ Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China 4​ State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, PR China.
5
5​ Microbial Resource Center, KRIBB, Daejeon 305-806, Republic of Korea.
6
6​ Bioproducts Research Chair (BRC), College of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia.

Abstract

A Gram-stain-negative, aerobic bacterium, designated strain YIM 77409T, was isolated from the Niujie hot spring in the Eryuan county of Dali, Yunnan province, south-west China. Cells of the strain were rod-shaped and colonies were yellow and circular. The strain grew at pH 6.0-8.0 (optimum, pH 7.0) and 50-70°C (optimum, 60-65°C). The predominant menaquinone was MK-8 and the DNA G+C content was 66.4 mol%. Major fatty acids (>10 %) were iso-C15 : 0 and iso-C17 : 0.The polar lipids consisted of one aminophospholipid, one phospholipid and two glycolipids. 16S rRNA gene sequence analysis showed that strain YIM 77409T formed a cluster with Thermus scotoductus DSM 8553T, Thermus antranikianii DSM 12462T, Thermus caliditerrae YIM 77925T and Thermus tengchongensis YIM 77924T, with highest 16S rRNA gene sequence similarity to T. scotoductus DSM 8553T (97.57%). However, DNA-DNA hybridization indicated that strain YIM 77409T should be viewed as a representative of a novel species, as there was only 30.6 ± 1.6% reassociation with T. scotoductus DSM 8553T. On the basis of the morphological and chemotaxonomic characteristics, as well as the genotypic data, it is proposed that strain YIM 77409T represents a novel species of the genus Thermus, with the name Thermus amyloliquefaciens sp. nov. The type strain is YIM 77409T ( = DSM 25898T = KCTC 32024T).

PMID:
25920724
DOI:
10.1099/ijs.0.000289
[Indexed for MEDLINE]

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