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Evid Based Complement Alternat Med. 2015;2015:739874. doi: 10.1155/2015/739874. Epub 2015 Apr 2.

Antiproliferative and Apoptosis-Inducing Activities of 4-Isopropyl-2,6-bis(1-phenylethyl)phenol Isolated from Butanol Fraction of Cordyceps bassiana.

Author information

1
Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
2
Department of Chemistry, Kwangwoon University, Seoul 139-701, Republic of Korea.
3
Institute for Bio-Medical Convergence, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University, Incheon 404-834, Republic of Korea.
4
Department of Biochemistry, Kangwon National University, Chuncheon 220-700, Republic of Korea.
5
Department of Life Science, Gachon University, Seongnam, Kyeonggi-do 461-701, Republic of Korea.
6
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea.
7
Department of Chemistry, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
8
Mushroom Research Division, Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA, Suwon 441-707, Republic of Korea.
9
Department of Biochemistry, Kangwon National University, Chuncheon 200-701, Republic of Korea.

Abstract

The Cordyceps species have been widely used for treating various cancer diseases. Although the Cordyceps species have been widely known as an alternative anticancer remedy, which compounds are responsible for their anticancer activity is not fully understood. In this study, therefore, we examined the anticancer activity of 5 isolated compounds derived from the butanol fraction (Cb-BF) of Cordyceps bassiana. For this purpose, several cancer cell lines such as C6 glioma, MDA-MB-231, and A549 cells were employed and details of anticancer mechanism were further investigated. Of 5 compounds isolated by activity-guided fractionation from BF of Cb-EE, KTH-13, and 4-isopropyl-2,6-bis(1-phenylethyl)phenol, Cb-BF was found to be the most potent antiproliferative inhibitor of C6 glioma and MDA-MB-231 cell growth. KTH-13 treatment increased DNA laddering, upregulated the level of Annexin V positive cells, and altered morphological changes of C6 glioma and MDA-MB-231 cells. In addition, KTH-13 increased the levels of caspase 3, caspase 7, and caspase 9 cleaved forms as well as the protein level of Bax but not Bcl-2. It was also found that the phosphorylation of AKT and p85/PI3K was also clearly reduced by KTH-13 exposure. Therefore, our results suggest KTH-13 can act as a potent antiproliferative and apoptosis-inducing component from Cordyceps bassiana, contributing to the anticancer activity of this mushroom.

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