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Fish Shellfish Immunol. 2015 Aug;45(2):293-9. doi: 10.1016/j.fsi.2015.04.024. Epub 2015 Apr 24.

MiR-31 modulates coelomocytes ROS production via targeting p105 in Vibrio splendidus challenged sea cucumber Apostichopus japonicus in vitro and in vivo.

Author information

1
School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315211, PR China.
2
School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315211, PR China. Electronic address: lichenghua@nbu.edu.cn.

Abstract

MiR-31 is a critical regulator of gene expression in many pathogenic processes in vertebrates. In this study, we identified p105 as a novel target of miR-31 in Apostichopus japonicus and investigated their regulatory roles in vitro and in vivo. The negative expression profiles between miR-31 and Ajp105 were detected in both LPS-exposed primary coelomocytes and Vibrio splendidus-challenged sea cucumber. Co-infection miR-31 mimics significantly depressed the expression of Ajp105 and increased ROS production in vitro. In contrast, miR-31 inhibitor significantly elevated the expression of Ajp105 and decreased ROS level. Consistently, miR-31 over-expression or Ajp105 silencing in vivo both greatly promoted ROS accumulation. Taken together, our findings confirmed that miR-31 could modulate respiratory burst via targeting Ajp105 during sea cucumber pathological development.

KEYWORDS:

Apostichopus japonicus; MiR-31; Respiratory burst; p105

PMID:
25917973
DOI:
10.1016/j.fsi.2015.04.024
[Indexed for MEDLINE]

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