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Infect Genet Evol. 2015 Jul;33:101-9. doi: 10.1016/j.meegid.2015.04.017. Epub 2015 Apr 24.

A molecular phylogenetics-based approach for identifying recent hepatitis C virus transmission events.

Author information

1
BC Centre for Disease Control, Vancouver, BC, Canada; University of British Columbia, Vancouver, BC, Canada. Electronic address: andrea.olmstead@bccdc.ca.
2
BC Centre for Excellence in HIV/AIDS, St Paul's Hospital, Vancouver, BC, Canada.
3
BC Centre for Disease Control, Vancouver, BC, Canada; University of British Columbia, Vancouver, BC, Canada.
4
University of British Columbia, Vancouver, BC, Canada.
5
The Kirby Institute, UNSW Australia, Sydney, NSW, Australia.
6
University of British Columbia, Vancouver, BC, Canada; BC Centre for Excellence in HIV/AIDS, St Paul's Hospital, Vancouver, BC, Canada.
7
Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
8
BC Centre for Disease Control, Vancouver, BC, Canada.

Abstract

Improved surveillance methods are needed to better understand the current hepatitis C virus (HCV) disease burden and to monitor the impact of prevention and treatment interventions on HCV transmission dynamics. Sanger sequencing (HCV NS5B, HVR1 and Core-E1-HVR1) and phylogenetics were applied to samples from individuals diagnosed with HCV in British Columbia, Canada in 2011. This included individuals with two or three sequential samples collected <1 year apart. Patristic distances between sequential samples were used to set cutoffs to identify recent transmission clusters. Factors associated with transmission clustering were analyzed using logistic regression. From 618 individuals, 646 sequences were obtained. Depending on the cutoff used, 63 (10%) to 92 (15%) unique individuals were identified within transmission clusters of predicted recent origin. Clustered individuals were more likely to be <40 years old (Adjusted Odds Ratio (AOR) 2.12, 95% CI 1.21-3.73), infected with genotype 1a (AOR 6.60, 95% CI 1.98-41.0), and to be seroconverters with estimated infection duration of <1 year (AOR 3.13, 95% CI 1.29-7.36) or >1 year (AOR 2.19, 95% CI 1.22-3.97).

CONCLUSION:

Systematic application of molecular phylogenetics may be used to enhance traditional surveillance methods through identification of recent transmission clusters.

KEYWORDS:

British Columbia; Epidemiology; Hepatitis C virus; Phylogenetics; Recent transmission clusters; Sequencing; Seroconversion; Surveillance

PMID:
25917496
DOI:
10.1016/j.meegid.2015.04.017
[Indexed for MEDLINE]
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