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Am J Reprod Immunol. 2015 Oct;74(4):302-8. doi: 10.1111/aji.12388. Epub 2015 Apr 28.

Priming with DNA Enhances Considerably the Immunogenicity of hCG β-LTB Vaccine.

Author information

1
The Talwar Research Foundation, New Delhi, India.
2
Jamia Hamdard, New Delhi, India.
3
National Institute of Immunology, New Delhi, India.

Abstract

PROBLEM:

Necessity to elicit antibody response above the protective threshold titres by sexually active women immunized to prevent pregnancy.

METHOD OF STUDY:

Recombinant hCGβ-LTB vaccine expressed as both DNA and protein. Balb C mice employed for testing immunogenicity.

RESULTS:

Necessity to give three primary injections of the vaccine to elicit proper antibody response. Immunization twice with DNA form of the vaccine at fortnightly interval followed by the protein elicits a distinctly higher antibody response than proteinic vaccine alone. Antibodies generated are bio-effective against hCG.

CONCLUSION:

Immunization with the DNA form of the recombinant hCGβ-LTB vaccine twice at fortnightly interval followed by the proteinic form of the vaccine induces distinctly higher antibody response.

KEYWORDS:

DNA Vaccine; Mycobacterium indicus pranii (MiP); human chorionic gonadotropin (hCG)

PMID:
25917014
DOI:
10.1111/aji.12388
[Indexed for MEDLINE]

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