A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells

Methods Mol Biol. 2015:1300:1-12. doi: 10.1007/978-1-4939-2596-4_1.

Abstract

High-throughput imaging of yeast cells expressing fluorescent proteins can be used to understand biological pathways in the context of spatial organization. Here we describe a method for imaging yeast cells expressing proteins tagged with green fluorescent protein (GFP) and/or red fluorescent protein (RFP), with or without drug treatment, in a 384-well format, using the PerkinElmer Opera high-content confocal imaging microscope.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • DNA Replication*
  • Imaging, Three-Dimensional
  • Microscopy, Confocal / methods*
  • Microscopy, Fluorescence / methods*
  • Saccharomyces cerevisiae / cytology*
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism*
  • Stress, Physiological*
  • Time Factors