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Methods. 2015 Oct 1;87:31-45. doi: 10.1016/j.ymeth.2015.04.015. Epub 2015 Apr 24.

Integrated systems for exosome investigation.

Author information

1
System Biosciences (SBI), 265 N. Whisman Rd., Mountain View, CA 94043, USA.
2
System Biosciences (SBI), 265 N. Whisman Rd., Mountain View, CA 94043, USA. Electronic address: tantes@systembio.com.

Abstract

Extracellular vesicles, including exosomes, are currently being investigated to better understand their biogenesis and biological functions. There is also a rapidly growing interest in utilizing exosomes present in patient biofluids for molecular diagnostics in the clinic. Exosomes are natural shuttles of RNA and protein cargo, making them attractive as potential therapeutic delivery vehicles. Here, we describe the methods for using the latest tools and technologies to study exosomes to better understand their roles in cell-to-cell communication, for discovery of clinical biomarkers and to engineer exosomes for therapeutic applications.

KEYWORDS:

Exosomes; Extracellular vesicles; Liquid biopsy; Nanobiomedicine; Oncosomes; miRNA biomarkers

PMID:
25916618
DOI:
10.1016/j.ymeth.2015.04.015
[Indexed for MEDLINE]

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