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Nat Methods. 2015 Jun;12(6):519-22. doi: 10.1038/nmeth.3370. Epub 2015 Apr 27.

G&T-seq: parallel sequencing of single-cell genomes and transcriptomes.

Author information

1
Sanger Institute-EBI Single-Cell Genomics Centre, Wellcome Trust Sanger Institute, Hinxton, UK.
2
MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
3
Department of Human Genetics, University of Leuven, Leuven, Belgium.
4
Cancer Genome Project, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
5
Department of Physiology, Development and Neuroscience, Downing Site, University of Cambridge, Cambridge, UK.
6
Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK.
7
Sequencing R&D, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
8
Cytogenetics Core Facility, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
9
1] Sanger Institute-EBI Single-Cell Genomics Centre, Wellcome Trust Sanger Institute, Hinxton, UK. [2] MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
10
1] Sanger Institute-EBI Single-Cell Genomics Centre, Wellcome Trust Sanger Institute, Hinxton, UK. [2] Department of Human Genetics, University of Leuven, Leuven, Belgium.

Abstract

The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.

PMID:
25915121
DOI:
10.1038/nmeth.3370
[Indexed for MEDLINE]

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