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Scanning. 2015 Jul-Aug;37(4):246-57. doi: 10.1002/sca.21205. Epub 2015 Apr 23.

Chrysin-organogermanium (IV) complex induced Colo205 cell apoptosis-associated mitochondrial function and anti-angiogenesis.

Yang F1,2, Gong L3, Jin H1,2, Pi J1,2, Bai H1, Wang H3, Cai H1, Yang P1, Cai J1,2.

Author information

1
Department of Chemistry of Jinan University, Guangzhou, China.
2
State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China.
3
Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou, China.

Abstract

Colorectal cancer, a kind of malignant cancer, has more than 1 million new patients and results in 0.5 million deaths every year globally based on the estimation of Globocan in 2008. One of the most important issues against colon cancer is tumor metastasis. Anti-angiogenesis, a form of targeted therapy uses drugs or other substances to prevent the new blood vessel formation, which is critical for tumor metastasis. In our previous studies, we have demonstrated a simple method to synthesize Chry-Ge complex through the reaction between chrysin and triphenylgermanium bromide. In this work, we investigated the mechanism of Chry-Ge induced Colo205 cell apoptosis. We found that Chry-Ge could induce apoptosis in Colo205 cells in mitochondrial-dependent pathway, cause the reorganization of cytoskeleton and induce the damage of nucleus in Colo205 cells. Besides, Chry-Ge was also found to induce membrane ultrastructural changes in Colo205 cells by AFM. Further, we found that Chry-Ge can inhibit tube formation of human umbilical vascular endothelial cell in vitro. Chry-Ge was also tested in vivo in the chicken chorioallantoic membrane (CAM) assay and found to inhibit bFGF-treated CAMs development. These results suggested that Chry-Ge could induce Colo205 cell apoptosis by mitochondrial pathway and anti-angiogenesis, highlighting the use of organic germanium agents for the treatment of colorectal cancer.

KEYWORDS:

Chry-Ge; Chrysin; Colo205; atomic force microscopy (AFM); laser scanning confocal microscope (LSCM)

PMID:
25914235
DOI:
10.1002/sca.21205
[Indexed for MEDLINE]

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