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Clin Rheumatol. 2015 Oct;34(10):1807-11. doi: 10.1007/s10067-015-2947-5. Epub 2015 Apr 25.

Increased expression of IL-28RA mRNA in peripheral blood mononuclear cells from patients with systemic lupus erythematosus.

Author information

1
Institute of Dermatology and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Meishan Road, Hefei, 230032, Anhui, China.
2
Department of Dermatology, China-Japan Friendship Hospital, Beijing, 100029, China. wuhucuiyong@vip.163.com.
3
Institute of Dermatology and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Meishan Road, Hefei, 230032, Anhui, China. yangsen@medmail.com.cn.

Abstract

Systemic lupus erythematosus (SLE) is a systemic autoimmune and inflammatory disease with a strong genetic contribution and characterized by kinds of immune reactions. Our previous genome-wide association studies have identified IL-28RA as a susceptibility gene for SLE. In this study, we performed a quantitative reverse transcription polymerase chain reaction (RT-PCR) in 62 patients with SLE and 69 controls to investigate the different expression levels of IL-28RA in peripheral blood mononuclear cells (PBMCs) from SLE patients and healthy controls and the association between IL-28RA expression and systemic lupus erythematosus disease activity index (SLEDAI) or the variant of the single-nucleotide polymorphism (SNP) rs4649203. The expression levels of IL-28RA messenger RNA (mRNA) in SLE patients were significantly increased compared with those of healthy controls. In addition, there were also significant differences in the expression levels of IL-28RA between active (SLEDAI ≥ 6) or inactive (SLEDAI < 6) SLE groups and healthy controls. However, no correlation was observed between IL-28RA mRNA expression level and SLEDAI. There was no association between the variant of the SNP rs4649203 and IL-28RA mRNA expression levels neither. These results indicated that expression of IL-28RA mRNA may be correlated with the pathogenesis of SLE.

KEYWORDS:

IL-28RA; Quantitative reverse transcription polymerase chain reaction; Single-nucleotide polymorphism; Systemic lupus erythematosus

PMID:
25911176
DOI:
10.1007/s10067-015-2947-5
[Indexed for MEDLINE]

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