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Cell. 2015 Apr 23;161(3):450-7. doi: 10.1016/j.cell.2015.03.049.

Single-Particle Cryo-EM at Crystallographic Resolution.

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1
Department of Biochemistry and Biophysics, University of California San Francisco, 600 16th Street, San Francisco, CA 94158, USA. Electronic address: ycheng@ucsf.edu.

Abstract

Until only a few years ago, single-particle electron cryo-microscopy (cryo-EM) was usually not the first choice for many structural biologists due to its limited resolution in the range of nanometer to subnanometer. Now, this method rivals X-ray crystallography in terms of resolution and can be used to determine atomic structures of macromolecules that are either refractory to crystallization or difficult to crystallize in specific functional states. In this review, I discuss the recent breakthroughs in both hardware and software that transformed cryo-microscopy, enabling understanding of complex biomolecules and their functions at atomic level.

PMID:
25910205
PMCID:
PMC4409662
DOI:
10.1016/j.cell.2015.03.049
[Indexed for MEDLINE]
Free PMC Article
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