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FEBS Lett. 2015 May 22;589(12):1314-21. doi: 10.1016/j.febslet.2015.04.017. Epub 2015 Apr 20.

Protein phosphatase PP1-NIPP1 activates mesenchymal genes in HeLa cells.

Author information

1
Laboratory of Biosignaling & Therapeutics, KU Leuven Department of Cellular and Molecular Medicine, University of Leuven, B-3000 Leuven, Belgium.
2
Laboratory of Biosignaling & Therapeutics, KU Leuven Department of Cellular and Molecular Medicine, University of Leuven, B-3000 Leuven, Belgium. Electronic address: Aleyde.VanEynde@med.kuleuven.be.
3
Laboratory of Biosignaling & Therapeutics, KU Leuven Department of Cellular and Molecular Medicine, University of Leuven, B-3000 Leuven, Belgium. Electronic address: Mathieu.Bollen@med.kuleuven.be.

Abstract

The deletion of the protein phosphatase-1 (PP1) regulator known as Nuclear Inhibitor of PP1 (NIPP1) is embryonic lethal during gastrulation, hinting at a key role of PP1-NIPP1 in lineage specification. Consistent with this notion we show here that a mild, stable overexpression of NIPP1 in HeLa cells caused a massive induction of genes of the mesenchymal lineage, in particular smooth/cardiac-muscle and matrix markers. This reprogramming was associated with the formation of actin-based stress fibers and retracting filopodia, and a reduced proliferation potential. The NIPP1-induced mesenchymal transition required functional substrate and PP1-binding domains, suggesting that it involves the selective dephosphorylation of substrates of PP1-NIPP1.

KEYWORDS:

HeLa cells; Mesenchymal lineage; Nuclear inhibitor of PP1; Protein phosphatase-1

PMID:
25907536
DOI:
10.1016/j.febslet.2015.04.017
[Indexed for MEDLINE]
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