Format

Send to

Choose Destination
Biomaterials. 2015 Jun;54:34-43. doi: 10.1016/j.biomaterials.2015.03.003. Epub 2015 Mar 29.

A cyanine-modified upconversion nanoprobe for NIR-excited imaging of endogenous hydrogen peroxide signaling in vivo.

Author information

1
School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore.
2
Institute of Advanced Materials (IAM) and Jiangsu-Singapore Joint Research Center for Organic/Bio-Electronics & Information Displays, Nanjing Tech University (NanjingTech), Nanjing 211816, China.
3
College of Science, Nanjing Tech University (NanjingTech), Nanjing 211816, China.
4
School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore; Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, 637551, Singapore. Electronic address: JoachimLoo@ntu.edu.sg.
5
School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore; Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371, Singapore. Electronic address: qczhang@ntu.edu.sg.

Abstract

Endogenous hydrogen peroxide is an important parameter associated with cellular signal transduction and homeostasis. However, abnormal H2O2 regulation in live systems has been implicated in many pathological conditions. Monitoring this signal in live systems is essential but challenging because current H2O2 probes are impractical for efficient bio-imaging due to UV/visible light as the excitation source. We herein present a novel design based on an organic fluorophore-attached lanthanide-doped upconversion nanoprobe (CYD1-UCNPs) for selective UCL detection of H2O2. This nanoprobe represents the next-generation imaging tool that features a robust UCL "turn-on" response to H2O2 with NIR-excited ratiometric signals and has potential applications in ratiometric UCL imaging of endogenous H2O2 generating in living cells and whole-body animals.

KEYWORDS:

Hydrogen peroxide; Immune response; NIR-excited imaging; Ratiometric; Upconversion luminescence

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center