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Nat Commun. 2015 Apr 23;6:6807. doi: 10.1038/ncomms7807.

Direct neuronal glucose uptake heralds activity-dependent increases in cerebral metabolism.

Author information

1
1] Center for Translational Neuromedicine, University of Rochester, Rochester, New York 14642, USA [2] Faculty of Health and Medical Sciences, Center for Basic and Translational Neuroscience, University of Copenhagen, DK-2200 Copenhagen N, Denmark.
2
1] Center for Translational Neuromedicine, University of Rochester, Rochester, New York 14642, USA [2] Department of Clinical Pharmacology, China Medical University, 110001 Shenyang, China.

Abstract

Metabolically, the brain is a highly active organ that relies almost exclusively on glucose as its energy source. According to the astrocyte-to-neuron lactate shuttle hypothesis, glucose is taken up by astrocytes and converted to lactate, which is then oxidized by neurons. Here we show, using two-photon imaging of a near-infrared 2-deoxyglucose analogue (2DG-IR), that glucose is taken up preferentially by neurons in awake behaving mice. Anaesthesia suppressed neuronal 2DG-IR uptake and sensory stimulation was associated with a sharp increase in neuronal, but not astrocytic, 2DG-IR uptake. Moreover, hexokinase, which catalyses the first enzymatic steps in glycolysis, was highly enriched in neurons compared with astrocytes, in mouse as well as in human cortex. These observations suggest that brain activity and neuronal glucose metabolism are directly linked, and identify the neuron as the principal locus of glucose uptake as visualized by functional brain imaging.

PMID:
25904018
PMCID:
PMC4410436
DOI:
10.1038/ncomms7807
[Indexed for MEDLINE]
Free PMC Article

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