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Proc Natl Acad Sci U S A. 2015 May 5;112(18):5833-7. doi: 10.1073/pnas.1505811112. Epub 2015 Apr 20.

The resurrection genome of Boea hygrometrica: A blueprint for survival of dehydration.

Author information

1
School of Life Sciences, Capital Normal University, Beijing 100048, China;
2
Beijing Genomics Institute-Shenzhen, Shenzhen 518083, China;
3
Key Laboratory of Plant Resources and.
4
Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;
5
School of Life Sciences, University of Science and Technology of China, Hefei 230022, China;
6
Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai 200032, China; Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907; and jkzhu@purdue.edu Mel.Oliver@ars.usda.gov yhe@cnu.edu.cn.
7
Plant Genetics Research Unit, Midwest Area, Agricultural Research Service, United State Department of Agriculture, University of Missouri, Columbia, MO 65211 jkzhu@purdue.edu Mel.Oliver@ars.usda.gov yhe@cnu.edu.cn.
8
School of Life Sciences, Capital Normal University, Beijing 100048, China; jkzhu@purdue.edu Mel.Oliver@ars.usda.gov yhe@cnu.edu.cn.

Abstract

"Drying without dying" is an essential trait in land plant evolution. Unraveling how a unique group of angiosperms, the Resurrection Plants, survive desiccation of their leaves and roots has been hampered by the lack of a foundational genome perspective. Here we report the ∼1,691-Mb sequenced genome of Boea hygrometrica, an important resurrection plant model. The sequence revealed evidence for two historical genome-wide duplication events, a compliment of 49,374 protein-coding genes, 29.15% of which are unique (orphan) to Boea and 20% of which (9,888) significantly respond to desiccation at the transcript level. Expansion of early light-inducible protein (ELIP) and 5S rRNA genes highlights the importance of the protection of the photosynthetic apparatus during drying and the rapid resumption of protein synthesis in the resurrection capability of Boea. Transcriptome analysis reveals extensive alternative splicing of transcripts and a focus on cellular protection strategies. The lack of desiccation tolerance-specific genome organizational features suggests the resurrection phenotype evolved mainly by an alteration in the control of dehydration response genes.

KEYWORDS:

Boea hygrometrica; drought tolerance enhancement; genome; resurrection plant; vegetative desiccation tolerance

PMID:
25902549
PMCID:
PMC4426394
DOI:
10.1073/pnas.1505811112
[Indexed for MEDLINE]
Free PMC Article

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