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Asian Pac J Trop Med. 2015 Jan;8(1):48-52. doi: 10.1016/S1995-7645(14)60186-3.

Protective function of tocilizumab in human cardiac myocytes ischemia reperfusion injury.

Author information

1
Department of Cardiovascular Surgery, The Second Affiliated Hospital of Medical College of Zhejiang University, Hangzhou Zhejiang, 310009, China.
2
Department of Interventional cardiovascular center, The Second Affiliated Hospital of Medical College of Zhejiang University, Hangzhou Zhejiang, 310009, China.
3
Department of Cardiovascular Surgery, The Second Affiliated Hospital of Medical College of Zhejiang University, Hangzhou Zhejiang, 310009, China. Electronic address: kmj1956@163.com.

Abstract

OBJECTIVE:

To investigate the protective function of tocilizumab in human cardiac myocytes ischemia-reperfusion injury.

METHODS:

The human cardiac myocytes were treated by tocilizumab with different concentrations(1.0 mg/mL, 3.0 mg/mL, 5.0 mg/mL) for 24 h, then cells were cultured in ischemia environment for 24 h and reperfusion environment for 1 h. The MTT and flow cytometry were used to detect the proliferation and apoptosis of human cardiac myocytes, respectively. The mRNA and protein expressions of Bcl-2 and Bax were measured by qRT-PCR and western blot, respectively.

RESULTS:

Compared to the negative group, pretreated by tocilizumab could significantly enhance the proliferation viability and suppress apoptosis of human cardiac myocytes after suffering ischemia reperfusion injury (P<0.05). The expression of Bcl-2 in tocilizumab treated group were higher than NC group (P<0.05), while the Bax expression were lower (P<0.05).

CONCLUSIONS:

Tocilizumab could significantly inhibit apoptosis and keep the proliferation viability of human cardiac myocytes after suffering ischemia reperfusion injury. Tocilizumab may obtain a widely application in the protection of ischemia reperfusion injury.

KEYWORDS:

Human cardiac myocytes; Ischemia–reperfusion injury; Protection; Tocilizumab

PMID:
25901924
DOI:
10.1016/S1995-7645(14)60186-3
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