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Nucleic Acids Res. 2015 Aug 18;43(14):e93. doi: 10.1093/nar/gkv366. Epub 2015 Apr 19.

Single-cell, locus-specific bisulfite sequencing (SLBS) for direct detection of epimutations in DNA methylation patterns.

Author information

1
Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA silvia.gravina@einstein.yu.edu.
2
Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Abstract

Stochastic epigenetic changes drive biological processes, such as development, aging and disease. Yet, epigenetic information is typically collected from millions of cells, thereby precluding a more precise understanding of cell-to-cell variability and the pathogenic history of epimutations. Here we present a novel procedure for directly detecting epimutations in DNA methylation patterns using single-cell, locus-specific bisulfite sequencing (SLBS). We show that within gene promoter regions of mouse hepatocytes the epimutation rate is two orders of magnitude higher than the mutation rate.

PMID:
25897117
PMCID:
PMC4538804
DOI:
10.1093/nar/gkv366
[Indexed for MEDLINE]
Free PMC Article

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