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Nat Immunol. 2015 Jun;16(6):663-73. doi: 10.1038/ni.3154. Epub 2015 Apr 20.

Transcriptional profiling of mouse B cell terminal differentiation defines a signature for antibody-secreting plasma cells.

Author information

1
1] The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia. [2] Department of Computing and Information Systems, The University of Melbourne, Parkville, Victoria, Australia.
2
1] The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia. [2] Department of Medical Biology, The University of Melbourne, Parkville, Victoria, Australia.
3
1] The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia. [2] Department of Pathology, The University of Melbourne, Parkville, Victoria, Australia. [3] Department of Microbiology &Immunology, The University of Melbourne, Parkville, Victoria, Australia.
4
1] The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia. [2] Department of Mathematics and Statistics, The University of Melbourne, Parkville, Victoria, Australia.

Abstract

When B cells encounter an antigen, they alter their physiological state and anatomical localization and initiate a differentiation process that ultimately produces antibody-secreting cells (ASCs). We have defined the transcriptomes of many mature B cell populations and stages of plasma cell differentiation in mice. We provide a molecular signature of ASCs that highlights the stark transcriptional divide between B cells and plasma cells and enables the demarcation of ASCs on the basis of location and maturity. Changes in gene expression correlated with cell-division history and the acquisition of permissive histone modifications, and they included many regulators that had not been previously implicated in B cell differentiation. These findings both highlight and expand the core program that guides B cell terminal differentiation and the production of antibodies.

PMID:
25894659
DOI:
10.1038/ni.3154
[Indexed for MEDLINE]

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