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Neuron. 2015 May 6;86(3):672-9. doi: 10.1016/j.neuron.2015.03.050. Epub 2015 Apr 16.

Mobility of calcium channels in the presynaptic membrane.

Author information

1
Molecular Physiology Group, Leibniz Institute for Neurobiology, D-39118 Magdeburg, Germany.
2
University of Bordeaux, F-33000 Bordeaux, France; CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France.
3
Harvard University, Department Molecular and Cellular Biology, Cambridge, MA 02138, USA.
4
Molecular Physiology Group, Leibniz Institute for Neurobiology, D-39118 Magdeburg, Germany; Presynaptic Plasticity Group, Leibniz Institute for Neurobiology, D-39118 Magdeburg, Germany.
5
University of Bordeaux, F-33000 Bordeaux, France; CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France; Bordeaux Imaging Center, UMS 3420 CNRS, US4 INSERM, University of Bordeaux, F-33000 Bordeaux, France.
6
Department Neurochemistry, Leibniz Institute for Neurobiology, D-39118 Magdeburg, Germany.
7
Otto-von-Guericke-University Magdeburg, Systemverfahrenstechnik, Universitätsplatz 2, D-39106 Magdeburg, Germany.
8
Molecular Physiology Group, Leibniz Institute for Neurobiology, D-39118 Magdeburg, Germany. Electronic address: martin.heine@lin-magdeburg.de.

Abstract

Unravelling principles underlying neurotransmitter release are key to understand neural signaling. Here, we describe how surface mobility of voltage-dependent calcium channels (VDCCs) modulates release probabilities (P(r)) of synaptic vesicles (SVs). Coupling distances of <10 to >100 nm have been reported for SVs and VDCCs in different synapses. Tracking individual VDCCs revealed that within hippocampal synapses, ∼60% of VDCCs are mobile while confined to presynaptic membrane compartments. Intracellular Ca(2+) chelation decreased VDCC mobility. Increasing VDCC surface populations by co-expression of the α2δ1 subunit did not alter channel mobility but led to enlarged active zones (AZs) rather than higher channel densities. VDCCs thus scale presynaptic scaffolds to maintain local mobility. We propose that dynamic coupling based on mobile VDCCs supports calcium domain cooperativity and tunes neurotransmitter release by equalizing Pr for docked SVs within AZs.

PMID:
25892305
DOI:
10.1016/j.neuron.2015.03.050
[Indexed for MEDLINE]
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