Format

Send to

Choose Destination
Cytokine. 2015 Aug;74(2):335-8. doi: 10.1016/j.cyto.2015.03.016. Epub 2015 Apr 15.

Resveratrol attenuates CXCL11 expression induced by proinflammatory cytokines in retinal pigment epithelial cells.

Author information

1
Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, United States. Electronic address: kuttyk@nei.nih.gov.
2
Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, United States.
3
Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, United States.
4
National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, United States.

Abstract

Dysfunction of the retinal pigment epithelium (RPE) resulting from chronic inflammation is implicated in the pathogenesis of age-related macular degeneration (AMD). RPE cells adjacent to drusen deposits in the AMD eye are known to contain CXCL11, a chemokine involved in inflammatory cell recruitment. We investigated the CXCL11 production by the human RPE (ARPE-19) cells under inflammatory conditions and tested its response to resveratrol, a naturally occurring anti-inflammatory antioxidant. A proinflammatory cytokine mixture consisting of IFN-γ, IL-1β and TNF-α highly increased CXCL11 mRNA expression and CXCL11 protein secretion by ARPE-19 cells. Resveratrol substantially inhibited the proinflammatory cytokines-induced CXCL11 production while partially blocking nuclear factor-κB activation. This inhibitory action of resveratrol was also observed for the cytokines-induced expression of chemokines CXCL9, CCL2 and CCL5. Our results indicate that resveratrol could potentially attenuate RPE inflammatory response implicated in the pathogenesis of AMD.

KEYWORDS:

Age-related macular degeneration; CXCL11; Nuclear factor-kappa B; Resveratrol; Retinal pigment epithelium

PMID:
25890876
PMCID:
PMC4475468
DOI:
10.1016/j.cyto.2015.03.016
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center